Scientists at SBC and APS received an ANL impact award on its 2020 research encompassing the SARS CoV-2 virus and continues to research for therapeutics and treatments to combat infectious disease.
The APS uses advanced instrumentation, state-of-the-art software and methods and high throughput technologies. SBC, now eBERlight is capable of addressing the most challenging projects in structural biology that include: large macromolecular assemblies (ribosomes, viruses, regulatory protein/DNAcomplexes), membrane-bound and membrane-associated proteins (ion channels, receptors, integrins). Structures of enzymes and complexes with ligands and inhibitors can be determined at atomic resolution. Thanks to recent advances, faster detectors, and automation, one does not need not be a macromolecular crystallographer to take advantage of these facilities; our experienced staff are available to guide even novice users through the entire process.
Publications Highlights
Structure and enzymatic characterization of CelD endoglucanase from the anaerobic fungus Piromyces finnis. Appl Microbiol Biotechnol. 2023 Oct;107(19):5999-6011. doi: 10.1007/s00253-023-12684-0. Epub 2023 Aug 7.PMID: 37548665
Structural basis of impaired disaggregase function in the oxidation-sensitive SKD3 mutant causing 3-methylglutaconic aciduria. Nat Commun. 2023 Apr 11;14(1):2028. doi: 10.1038/s41467-023-37657-9.PMID: 37041140
Dual domain recognition determines SARS-CoV-2 PLpro selectivity for human ISG15 and K48-linked di-ubiquitin. Wydorski PM, Osipiuk J, Lanham BT, Tesar C, Endres M, Engle E, Jedrzejczak R, Mullapudi V, Michalska K, Fidelis K, Fushman D, Joachimiak A, Joachimiak LA.Nat Commun. 2023 Apr 25;14(1):2366. doi: 10.1038/s41467-023-38031-5.PMID: 37185902
Sel1-like proteins and peptides are the major Oxalobacter formigenes-derived factors stimulating oxalate transport by human intestinal epithelial cells. Arvans D, Chang C, Alshaikh A, Tesar C, Babnigg G, Wolfgeher D, Kron S, Antonopoulos D, Bashir M, Cham C, Musch M, Chang E, Joachimiak A, Hassan H.Am J Physiol Cell Physiol. 2023 Jul 1;325(1):C344-C361. doi: 10.1152/ajpcell.00466.2021. Epub 2023 May 1.PMID: 37125773
Potent and selective covalent inhibition of the papain-like protease from SARS-CoV-2. Sanders BC, Pokhrel S, Labbe AD, Mathews II, Cooper CJ, Davidson RB, Phillips G, Weiss KL, Zhang Q, O'Neill H, Kaur M, Schmidt JG, Reichard W, Surendranathan S, Parvathareddy J, Phillips L, Rainville C, Sterner DE, Kumaran D, Andi B, Babnigg G, Moriarty NW, Adams PD, Joachimiak A, Hurst BL, Kumar S, Butt TR, Jonsson CB, Ferrins L, Wakatsuki S, Galanie S, Head MS, Parks JM.Nat Commun. 2023 Mar 28;14(1):1733. doi: 10.1038/s41467-023-37254-w.PMID: 36977673
A cryptic oxidoreductase safeguards oxidative protein folding in Corynebacterium diphtheriae. Reardon-Robinson ME, Nguyen MT, Sanchez BC, Osipiuk J, Rückert C, Chang C, Chen B, Nagvekar R, Joachimiak A, Tauch A, Das A, Ton-That H.Proc Natl Acad Sci U S A. 2023 Feb 21;120(8):e2208675120. doi: 10.1073/pnas.2208675120. Epub 2023 Feb 14.PMID: 36787356
A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae. Inniss NL, Kochan TJ, Minasov G, Wawrzak Z, Chang C, Tan K, Shuvalova L, Kiryukhina O, Pshenychnyi S, Wu R, Dubrovska I, Babnigg G, Endres M, Anderson WF, Hauser AR, Joachimiak A, Satchell KJF.Microbiol Resour Announc. 2023 Feb 16;12(2):e0101322. doi: 10.1128/mra.01013-22. Epub 2023 Jan 25.PMID: 36695589
Statistics by Year : BioSync (rcsb.org)
| SBC Sector 19 | 2021 | 2022 | 2023 | TOTAL | |||
|---|---|---|---|---|---|---|---|
| ID | BM | ID | BM | ID | BM | ||
| PDB Depositions | 148 | 36 | 124 | 44 | 11 | 3 | 6443 |
| 2021 | 148 | 36 |
| 2022 | 124 | 44 |
| 2023 | 11 | 3 |
| 6443 |
APS-U Capabilities with SBC and eBERlight
SBC is seeking collaborators interested in exploring other tools at the APS, such as macromolecular structural dynamics and imaging of soils, plants, or aerosol particulates. If you are interested, please contact eBERlight@anl.gov.
New opportunities are now available at SBC with Serial crystallography (SSX).
The APS-U will provide a highly brilliant beam ideally suited for serial crystallography methods. Serial crystallography methods have many advantages: they are more biologically relevant at room temperatures and reduce radiation damage by order of magnitude per structure. It also encourages the use of small or x-ray sensitive samples with the ability to perform time-resolved measurements. Advances in SSX experiments are paramount for the APS-U. This method is expected to flourish with the significant increase in x-ray flux density and a concomitant rise in multi-crystal datasets. If you are interested, please contact Karolina Michalska (kmichalska@anl.gov). Recent publication: 2'-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography.
The APCF will be open for users during the APS-U shutdown.
Submit an ESAF for Sector 84.