The Structural Biology Center (SBC) is a national user facility for macromolecular crystallography at the Advanced Photon Source. The facility uses advanced instrumentation, state-of-the-art software, and methods and high throughput technologies.

SBC continues to research for therapeutics and treatments to combat COVID-19. Scientists at SBC and APS received an ANL impact award on its 2020 research encompassing the SARS CoV-2 virus.

The facility uses advanced instrumentation, state-of-the-art software and methods and high throughput technologies. SBC is capable of addressing the most challenging projects in structural biology that include: large macromolecular assemblies (ribosomes, viruses, regulatory protein/DNAcomplexes), membrane-bound and membrane-associated proteins (ion channels, receptors, integrins). Structures of enzymes and complexes with ligands and inhibitors can be determined at atomic resolution.

The Structural Biology Center enables the atomic-scale study of macromolecular systems using very small crystal samples. It also offers the most efficient data collection and structure determination systems for protein crystallography worldwide. Thanks to recent advances with larger, faster X-ray detectors and automation of laboratory processes for expressing proteins and growing crystals, the time required to solve molecular structures has been greatly reduced. Research that not long ago took months or years may now take only hours. In addition, one need not be a macromolecular crystallographer to take advantage of these facilities; our experienced beamline staff are available to guide even novice users through the entire process.



Publications Highlights

Tipiracil binds to uridine site and inhibits Nsp15 endoribonuclease NendoU from SARS-CoV-2. Kim Y, Wower J, Maltseva N, Chang C, Jedrzejczak R, Wilamowski M, Kang S, Nicolaescu V, Randall G, Michalska K, Joachimiak A.Commun Biol. 2021 Feb 9;4(1):193. doi: 10.1038/s42003-021-01735-9.PMID: 33564093

Room-temperature X-ray crystallography reveals the oxidation and reactivity of cysteine residues in SARS-CoV-2 3CL Mpro: insights into enzyme mechanism and drug design. Kneller DW, Phillips G, O'Neill HM, Tan K, Joachimiak A, Coates L, Kovalevsky A.IUCrJ. 2020 Sep 21;7(Pt 6):1028-35. doi: 10.1107/S2052252520012634. Online ahead of print.PMID: 33063790

Structure of papain-like protease from SARS-CoV-2 and its complexes with non-covalent inhibitors.Osipiuk J, Azizi SA, Dvorkin S, Endres M, Jedrzejczak R, Jones KA, Kang S, Kathayat RS, Kim Y, Lisnyak VG, Maki SL, Nicolaescu V, Taylor CA, Tesar C, Zhang YA, Zhou Z, Randall G, Michalska K, Snyder SA, Dickinson BC, Joachimiak A.  Nat Commun. 2021 Feb 2;12(1):743. doi: 10.1038/s41467-021-21060-3. PMID: 33531496

Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein. Hodge CD, Rosenberg DJ, Wilamowski M, Joachimiak A , Hura GL, Hammel M. bioRxiv. 2021 Jan 13:2021.01.13.426597. doi: 10.1101/2021.01.13.426597. Preprint. PMID: 33469584

SARS-CoV-2 Infection Severity Is Linked to Superior Humoral Immunity against the Spike. Guthmiller JJ, Stovicek O, Wang J, Changrob S, Li L, Halfmann P, Zheng NY, Utset H, Stamper CT, Dugan HL, Miller WD, Huang M, Dai YN, Nelson CA, Hall PD, Jansen M, Shanmugarajah K, Donington JS, Krammer F, Fremont DH, Joachimiak A , Kawaoka Y, Tesic V, Madariaga ML, Wilson PC.  mBio. 2021 Jan 19;12(1):e02940-20. doi: 10.1128/mBio.02940-20. PMID: 33468695

Therapeutic genetic variation revealed in diverse Hsp104 homologs.March ZM, Sweeney K, Kim H, Yan X, Castellano LM, Jackrel ME, Lin J, Chuang E, Gomes E, Willicott CW, Michalska K, Jedrzejczak RP, Joachimiak A , Caldwell KA, Caldwell GA, Shalem O, Shorter J. Elife. 2020 Dec 15;9:e57457. doi: 10.7554/eLife.57457. PMID: 33319748

Distinct B cell subsets give rise to antigen-specific antibody responses against SARS-CoV-2.Wilson P, Stamper C, Dugan H, Li L, Asby N, Halfmann P, Guthmiller J, Zheng NY, Huang M, Stovicek O, Wang J, Madariaga ML, Shanmugarajah K, Jansen M, Amanat F, Stewart I, Changrob S, Utset H, Huang J, Nelson C, Dai YN, Hall P, Jedrzejczak R, Joachimiak A, Krammer F, Fremont D, Kawaoka Y.  Res Sq. 2020 Sep doi: 10.21203/ Preprint. PMID: 32995763

Crystal structures of SARS-CoV-2 ADP-ribose phosphatase: from the apo form to ligand complexes.Michalska K, Kim Y, Jedrzejczak R, Maltseva NI, Stols L, Endres M, Joachimiak A.  IUCrJ. 2020 Jul 17;7(Pt 5):814-824. doi: 10.1107/S2052252520009653. eCollection 2020 Sep 1. PMID: 32939273

SARS-CoV-2 infection severity is linked to superior humoral immunity against the spike.Guthmiller JJ, Stovicek O, Wang J, Changrob S, Li L, Halfmann P, Zheng NY, Utset H, Stamper CT, Dugan HL, Miller WD, Huang M, Dai YN, Nelson CA, Hall PD, Jansen M, Shanmugarajah K, Donington JS, Krammer F, Fremont DH, Joachimiak A  , Kawaoka Y, Tesic V, Madariaga ML, Wilson PC. bioRxiv. 2020 Sep 13:2020.09.12.294066. doi: 10.1101/2020.09.12.294066. Preprint. PMID: 32935099 

Structural plasticity of SARS-CoV-2 3CL Mpro active site cavity revealed by room temperature X-ray crystallography.Kneller DW, Phillips G, O'Neill HM, Jedrzejczak R, Stols L, Langan P, Joachimiak A  , Coates L, Kovalevsky A. Nat Commun. 2020 Jun 24;11(1):3202. doi: 10.1038/s41467-020-16954-7. PMID: 32581217

The crystal structure of nsp10-nsp16 heterodimer from SARS-CoV-2 in complex with S-adenosylmethionine.Rosas-Lemus M, Minasov G, Shuvalova L, Inniss NL, Kiryukhina O, Wiersum G, Kim Y, Jedrzejczak R, Maltseva NI, Endres M, Jaroszewski L, Godzik A, Joachimiak A, Satchell KJF. bioRxiv. 2020 Apr 26:2020.04.17.047498. doi: 10.1101/2020.04.17.047498. Preprint. PMID: 32511376




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