Scientists at SBC and APS received an ANL impact award on its 2020 research encompassing the SARS CoV-2 virus and continues to research for therapeutics and treatments to combat infectious disease.
The APS uses advanced instrumentation, state-of-the-art software and methods and high throughput technologies. SBC, now eBERlight is capable of addressing the most challenging projects in structural biology that include: large macromolecular assemblies (ribosomes, viruses, regulatory protein/DNAcomplexes), membrane-bound and membrane-associated proteins (ion channels, receptors, integrins). Structures of enzymes and complexes with ligands and inhibitors can be determined at atomic resolution. Thanks to recent advances, faster detectors, and automation, one does not need not be a macromolecular crystallographer to take advantage of these facilities; our experienced staff are available to guide even novice users through the entire process.
Publications Highlights
Priyanka Gade, Changsoo Chang, Denise S. Pryde, Daniel Fletcher, Sarah Niven, Luma Godoy Magalhaes, David Robinson, Jagmohan Saini, Peter E.G.F. Ibrahim, Barbara Forte, Jacek Wower, Michael J. Bodkin, Beatriz Baragaña, Ian H. Gilbert, Karolina Michalska, Andrzej Joachimiak, "Different chemical scaffolds bind to L-phe site in Mycobacterium tuberculosis Phe-tRNA synthetase," Eur. J. Med. Chem. 287, 117335 (2025). DOI: 10.1016/j.ejmech.2025.117335
Nicole L. Inniss, George Minasov, Changsoo Chang, Kemin Tan, Youngchang Kim, Natalia Maltseva, Peter Stogios, Ekaterina Filippova, Karolina Michalska, Jerzy Osipiuk, Lukasz Jaroszewki, Adam Godzik, Alexei Savchenko, Andrzej Joachimiak, Wayne F. Anderson, Karla J.F. Satchell, "Structural genomics of bacterial drug targets: Application of a high-throughput pipeline to solve 58 protein structures from pathogenic and related bacteria," Microbiology 14 (6), e00200 (2025). DOI: 10.1128/mra.00200-25
Claudia E. Mayer-Harnisch, Daniel Figueroa Paniagua, Natalia Maltseva, Youngchang Kim, Van Thi Bich Le, Andrzej Joachimiak, Misty L. Kuhn, "N-terminal domain swapping: A new paradigm for spermidine/spermine N-acetyltransferase (SSAT) protein structures?," Biochem. Biophys. Res. Commun. 748, 151302 (2025). DOI: 10.1016/j.bbrc.2025.151302
Xingyou Wang, Masha M. Rosenberg, Youngchang Kim, Natalia Maltseva, Gregory D. Cuny, Andrzej Joachimiak, Petr Kuzmič, Lizbeth Hedstrom, "Role of the Mobile Active Site Flap in IMP Dehydrogenase Inhibitor Binding," ACS Infect. Dis. 11 (2), 442-452 (2025). DOI: 10.1021/acsinfecdis.4c00636
Kartik Sachar, Katarzyna Kanarek, Jake Colautti, Youngchang Kim, Eran Bosis, Gerd Prehna, Dor Salomon, John C. Whitney, "A conserved chaperone protein is required for the formation of a noncanonical type VI secretion system spike tip complex," J. Biol. Chem. 301 (3), 108242 (2025). DOI: 10.1016/j.jbc.2025.108242
Julian C.-H. Chen, Miroslaw Gilski, Changsoo Chang, Dominika Borek, Gerd Rosenbaum, Alex Lavens, Zbyszek Otwinowski, Maciej Kubicki, Zbigniew Dauter, Mariusz Jaskolskid, Andrzej Joachimiak, "Solvent organization in the ultrahigh-resolution crystal structure of crambin at room temperature," IUCrJ 11 (5), 649-663 (2024). DOI: 10.1107/S2052252524007784
Statistics by Year : BioSync (rcsb.org)
| 2024 | 2025 | 2026 | TOTAL | ||||
|---|---|---|---|---|---|---|---|
| SBC Sector 19 | BM | ID | BM | ID | BM | ID | |
| PDB Depositions | 19 | 72 | 3 | 23 | 1 | 0 | 6635 |
| 2024 | 72 | 19 |
| 2025 | 23 | 3 |
| 2026 | 0 | 1 |
| 6635 |
APS-U Capabilities
New opportunities are now available at eBERlight
The APS-U will provide a highly brilliant beam ideally suited for serial crystallography methods. Serial crystallography methods have many advantages: they are more biologically relevant at room temperatures and reduce radiation damage by order of magnitude per structure. It also encourages the use of small or x-ray sensitive samples with the ability to perform time-resolved measurements. Advances in SSX experiments are paramount for the APS-U. This method is expected to flourish with the significant increase in x-ray flux density and a concomitant rise in multi-crystal datasets. If you are interested, please contact [email protected].
The Advanced Protein Characterization Facility (APCF) is open for users. This state-of-the-art laboratory has a HTP structural biology pipeline composed of bioinformatics, robotic gene cloning, protein expression, purification, characterization, crystallization, and structure determination.
To gain access, register with the user office and submit an ESAF for Sector 84.